BL21 Rosetta2 ΔrecBCD
(Bacterial strain
#176583)
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PurposeE. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.
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Depositing Lab
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Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 176583 | Bacteria in agar stab | 1 | $85 | |
Backbone
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Vector backboneStrain
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Modifications to backboneThis strain is a derivative of BL21 ΔrecBCD. It was made by re-transformation with pRARE2 plasmid.
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 12.5 μg/mL
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Growth Temperature37°C
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Growth Strain(s)BL21 ΔrecBCD
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepRARE2 plasmid
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MutationrecBCD genomic deletion
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.09.07.459228v1 for bioRxiv preprint.
Primers for recBCD deletion verification:
Foward - ttgatttactgcccgagagc
Reverse - gtcaaccgaatgcagacatc
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
BL21 Rosetta2 ΔrecBCD was a gift from Jean-Loup Faulon (Addgene plasmid # 176583) -
For your References section:
Differentially Optimized Cell-Free Buffer Enables Robust Expression from Unprotected Linear DNA in Exonuclease-Deficient Extracts. Batista AC, Levrier A, Soudier P, Voyvodic PL, Achmedov T, Reif-Trauttmansdorff T, DeVisch A, Cohen-Gonsaud M, Faulon JL, Beisel CL, Bonnet J, Kushwaha M. ACS Synth Biol. 2022 Jan 16. doi: 10.1021/acssynbio.1c00448. 10.1021/acssynbio.1c00448 PubMed 35034449