pAB675
(Plasmid
#176578)
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PurposeExpressing three of the five genes of the n-butanol pathway.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176578 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRhokS_2
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namephaJ, ter, adhE2
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MutationNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAB675 was a gift from Arpita Bose (Addgene plasmid # 176578 ; http://n2t.net/addgene:176578 ; RRID:Addgene_176578) -
For your References section:
n-Butanol production by Rhodopseudomonas palustris TIE-1. Bai W, Ranaivoarisoa TO, Singh R, Rengasamy K, Bose A. Commun Biol. 2021 Nov 3;4(1):1257. doi: 10.1038/s42003-021-02781-z. 10.1038/s42003-021-02781-z PubMed 34732832
