pU-YLL058W-zr
(Plasmid
#176565)
-
PurposeExpression of YLL058W from Zygosaccharomyces rouxii for auxotrophic selection in the absence of uracil
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 176565 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepU
-
Vector typeYeast Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameYLL058W-zr
-
Alt nameHsu1 (SGD reserved)
-
Alt nameZYRO0B08338g
-
SpeciesZygosaccharomyces rouxii
-
Insert Size (bp)1721
-
MutationCodon optimised for expression in S.cerevisae
- Promoter TDH1(GAP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site AscI (not destroyed)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pU-YLL058W-zr was a gift from Markus Ralser (Addgene plasmid # 176565 ; http://n2t.net/addgene:176565 ; RRID:Addgene_176565)