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Addgene

pM-TagRFP657
(Plasmid #176563)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176563 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pM
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TagRFP657
  • Species
    Synthetic
  • Insert Size (bp)
    717
  • Promoter TDH1(GAP)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PacI (not destroyed)
  • 3′ cloning site AscI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains E38G and L304S mutations in MET17. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pM-TagRFP657 was a gift from Markus Ralser (Addgene plasmid # 176563 ; http://n2t.net/addgene:176563 ; RRID:Addgene_176563)

  • For your References section:

    Microbial communities form rich extracellular metabolomes that foster metabolic interactions and promote drug tolerance. Yu JSL, Correia-Melo C, Zorrilla F, Herrera-Dominguez L, Wu MY, Hartl J, Campbell K, Blasche S, Kreidl M, Egger AS, Messner CB, Demichev V, Freiwald A, Mulleder M, Howell M, Berman J, Patil KR, Alam MT, Ralser M. Nat Microbiol. 2022 Apr;7(4):542-555. doi: 10.1038/s41564-022-01072-5. Epub 2022 Mar 21. 10.1038/s41564-022-01072-5 PubMed 35314781
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