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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4730
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUBF
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SpeciesH. sapiens (human)
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Entrez GeneUBTF (a.k.a. CONDBA, NOR-90, UBF, UBF-1, UBF1, UBF2)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byD. Ploton, M.F. O'Donohue (Reims, France)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GFP-UBF was a gift from Tom Misteli (Addgene plasmid # 17656 ; http://n2t.net/addgene:17656 ; RRID:Addgene_17656) -
For your References section:
A kinetic framework for a mammalian RNA polymerase in vivo. Dundr M, Hoffmann-Rohrer U, Hu Q, Grummt I, Rothblum LI, Phair RD, Misteli T. Science. 2002 Nov 22. 298(5598):1623-6. 10.1126/science.1076164 PubMed 12446911