pM-mRuby2
(Plasmid
#176550)
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PurposeExpression of mRuby2 for auxotrophic selection in the absence of methionine
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176550 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepM
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameyomRuby2
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SpeciesSynthetic
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Insert Size (bp)714
- Promoter TDH1(GAP)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site AscI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains E38G and L304S mutations in MET17. These mutations are not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pM-mRuby2 was a gift from Markus Ralser (Addgene plasmid # 176550 ; http://n2t.net/addgene:176550 ; RRID:Addgene_176550) -
For your References section:
Microbial communities form rich extracellular metabolomes that foster metabolic interactions and promote drug tolerance. Yu JSL, Correia-Melo C, Zorrilla F, Herrera-Dominguez L, Wu MY, Hartl J, Campbell K, Blasche S, Kreidl M, Egger AS, Messner CB, Demichev V, Freiwald A, Mulleder M, Howell M, Berman J, Patil KR, Alam MT, Ralser M. Nat Microbiol. 2022 Apr;7(4):542-555. doi: 10.1038/s41564-022-01072-5. Epub 2022 Mar 21. 10.1038/s41564-022-01072-5 PubMed 35314781