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pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet
(Plasmid #176535)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176535 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCOLADuet1
  • Backbone size w/o insert (bp) 3719
  • Total vector size (bp) 6221
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
  • Species
    Delaware Bay metagenome
  • Insert Size (bp)
    2503

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet was a gift from Feng Zhang (Addgene plasmid # 176535 ; http://n2t.net/addgene:176535 ; RRID:Addgene_176535)

  • For your References section:

    The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F. Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. 10.1126/science.abj6856 PubMed 34591643
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