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pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep
(Plasmid #176534)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176534 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET45b(+)
  • Backbone size w/o insert (bp) 5062
  • Total vector size (bp) 8044
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRISPR-associated IscB (Delaware Bay metagenome)
  • Alt name
    IscB(HEARO)
  • Species
    E. coli, Brachypodium distachyon, Delaware Bay metagenome
  • Insert Size (bp)
    1098
  • GenBank ID
    WP_040064394.1
  • Promoter T7
  • Tags / Fusion Proteins
    • 14xHis-MBP-bdSUMO (N terminal on backbone)
    • TwinStrep (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep was a gift from Feng Zhang (Addgene plasmid # 176534 ; http://n2t.net/addgene:176534 ; RRID:Addgene_176534)

  • For your References section:

    The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases. Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F. Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. 10.1126/science.abj6856 PubMed 34591643
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