pLV-CMV-hXRCC1-mCherry-Hygro
(Plasmid #176532)

• Purpose: mCherry fused to the C-terminus of XRCC1 & a hygromycin resistance cassette
• Depositing Lab: Robert Sobol
• Publication: Wilk et al Sci Rep. 2020 Jan 20;10(1):651. doi: 10.1038/s41598-020-57506-9.

Backbone

• Vector backbone: Lentivirus
• Backbone manufacturer: VectorBuilder
• Total vector size (bp): 10941
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: X-Ray Repair Cross Complementing 1
• Alt name: XRCC1
• Species: H. sapiens (human)
• Insert Size (bp): 1899
• Entrez Gene: XRCC1 (a.k.a. RCC, SCAR26)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • 176525.pLV-CMV-hXRCC1-mCherry-Hygro.gb
• A portion of this plasmid was derived from a plasmid made by: VectorBuilder
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry