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pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP
(Plasmid #176531)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176531 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPBJ
  • Backbone size w/o insert (bp) 3100
  • Total vector size (bp) 10400
  • Vector type
    Mammalian Expression ; piggybac transposase integration vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    KGV transcription factor
  • Alt name
    KTR-Gal4-VP64
  • Species
    Synthetic
  • Insert Size (bp)
    1000
  • Tags / Fusion Proteins
    • ErkKTR (N terminal on insert)
    • Gal4 DNA binding domain (C terminal on insert)
    • VP64 transactivation domain (C terminal on insert)
    • PEST destabilization domain

Gene/Insert 2

  • Gene/Insert name
    TagBFP
  • Species
    Synthetic
  • Insert Size (bp)
    699

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP was a gift from Jared Toettcher (Addgene plasmid # 176531 ; http://n2t.net/addgene:176531 ; RRID:Addgene_176531)

  • For your References section:

    A synthetic gene circuit for imaging-free detection of signaling pulses. Ravindran PT, McFann S, Thornton RH, Toettcher JE. Cell Syst. 2022 Feb 16;13(2):131-142.e13. doi: 10.1016/j.cels.2021.10.002. Epub 2021 Nov 4. 10.1016/j.cels.2021.10.002 PubMed 34739875
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