Skip to main content
Addgene

pCRISPR01-FnCpf1
(Plasmid #176529)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176529 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pFC333
  • Backbone manufacturer
    Uffe Mortensen (Addgene plasmid # 87844)
  • Total vector size (bp) 12879
  • Vector type
    CRISPR ; Fungal Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FnCpf1
  • Species
    Synthetic; Francisella tularensis subsp. novicida U112
  • Insert Size (bp)
    3900
  • Promoter TEF1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site PmeI (unknown if destroyed)
  • 5′ sequencing primer Seq TEF F: 5'-GCCCAAGGTGGGAGTCTAGGAG
  • 3′ sequencing primer seq TEF R: 5'-CGCCTGGACGACTAAACCAAAATAGG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRISPR01-FnCpf1 was a gift from Kusol Pootanakit (Addgene plasmid # 176529 ; http://n2t.net/addgene:176529 ; RRID:Addgene_176529)

  • For your References section:

    Development of a CRISPR/Cpf1 system for targeted gene disruption in Aspergillus aculeatus TBRC 277. Abdulrachman D, Eurwilaichitr L, Champreda V, Chantasingh D, Pootanakit K. BMC Biotechnol. 2021 Feb 11;21(1):15. doi: 10.1186/s12896-021-00669-8. 10.1186/s12896-021-00669-8 PubMed 33573639
Related items:
Commonly requested with: