Skip to main content
Addgene

PEA1-Nuclease
(Plasmid #176528)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176528 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PEA1-Nuclease-Puro
  • Backbone manufacturer
    Addgene 171992
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
  • Species
    Synthetic
  • Mutation
    GC to CA point mutation in SpCas9 to restore nuclease function
  • Promoter CbH for Cas9, hU6 for gRNAs

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer --
  • 3′ sequencing primer BGH-reverse
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PEA1-Nuclease was a gift from Paul Thomas (Addgene plasmid # 176528 ; http://n2t.net/addgene:176528 ; RRID:Addgene_176528)

  • For your References section:

    Optimized nickase- and nuclease-based prime editing in human and mouse cells. Adikusuma F, Lushington C, Arudkumar J, Godahewa GI, Chey YCJ, Gierus L, Piltz S, Geiger A, Jain Y, Reti D, Wilson LOW, Bauer DC, Thomas PQ. Nucleic Acids Res. 2021 Sep 17. pii: 6371974. doi: 10.1093/nar/gkab792. 10.1093/nar/gkab792 PubMed 34534334
Related items: