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Addgene

GFP-HP1alpha
(Plasmid #17652)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17652 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBCHGN
  • Backbone manufacturer
    Zentgraf Lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HP1 alpha
  • Species
    H. sapiens (human)
  • Entrez Gene
    CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer GFP-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See author's map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-HP1alpha was a gift from Tom Misteli (Addgene plasmid # 17652 ; http://n2t.net/addgene:17652 ; RRID:Addgene_17652)

  • For your References section:

    Maintenance of stable heterochromatin domains by dynamic HP1 binding. Cheutin T, McNairn AJ, Jenuwein T, Gilbert DM, Singh PB, Misteli T. Science. 2003 Jan 31. 299(5607):721-5. 10.1126/science.1078572 PubMed 12560555
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