Cas12f-GE ver3.0
(Plasmid #176508)

• Purpose: Expresses Cas12f-GE in mammalian cells
• Depositing Lab: Yong-Sam Kim
• Publication: Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038/s41587-021-01009-z. doi: 10.1038/s41587-021-01009-z.

Backbone

• Vector backbone: px458-GFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: Cas12f-GE ver3.0
• Alt name: Cas14a-GE ver3.0
• Alt name: Un1Cas12f1-GE ver3.0
• Insert Size (bp): 1680
• Promoter: chicken β-actin

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: Cas12f-GE ver3.0
• Alt name: Cas14a-GE ver3.0
• Alt name: MS1/MS2/MS3 sgRNA modifications
• Insert Size (bp): 281
• Promoter: U6

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • plasmid ID 176508_ Cas12f-GE Ver3.0.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry