pSIR-bsr
(Plasmid
#176507)
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Purpose(Empty Backbone) Self-inactivating retrovirus vector containing a Blasticidin S-resistance gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176507 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSIR
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Backbone manufacturerClontech
- Backbone size (bp) 8089
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Modifications to backboneReplacement of the neomycin resistance gene with the Blasticidin S resistance gene
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Vector typeMammalian Expression, Retroviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSIR-bsr was a gift from Hodaka Fujii (Addgene plasmid # 176507 ; http://n2t.net/addgene:176507 ; RRID:Addgene_176507) -
For your References section:
pSIR-bsr, a self-inactivating retrovirus vector expressing the blasticidin S-resistance gene. Fujii H. Biol Methods Protoc. 2021 Dec 6;6(1):bpab022. doi: 10.1093/biomethods/bpab022. eCollection 2021. 10.1093/biomethods/bpab022 PubMed 34926829