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Addgene

3927-64
(Plasmid #176500)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176500 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCamR
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP-T2A-TFEB(human; delta30; amino acids 31-476)
  • Species
    H. sapiens (human)
  • Promoter TRE

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3927-64 was a gift from Albert La Spada (Addgene plasmid # 176500 ; http://n2t.net/addgene:176500 ; RRID:Addgene_176500)
  • For your References section:

    MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Hsu CL, Lee EX, Gordon KL, Paz EA, Shen WC, Ohnishi K, Meisenhelder J, Hunter T, La Spada AR. Nat Commun. 2018 Mar 5;9(1):942. doi: 10.1038/s41467-018-03340-7. 10.1038/s41467-018-03340-7 PubMed 29507340