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Addgene

3919-32
(Plasmid #176496)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176496 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCamR
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    puroR-T2A-TetOn3G
  • Promoter TRE

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC identified a P93S difference in the PuroR selectable marker that the depositing lab does not believe to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    3919-32 was a gift from Albert La Spada (Addgene plasmid # 176496 ; http://n2t.net/addgene:176496 ; RRID:Addgene_176496)

  • For your References section:

    MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Hsu CL, Lee EX, Gordon KL, Paz EA, Shen WC, Ohnishi K, Meisenhelder J, Hunter T, La Spada AR. Nat Commun. 2018 Mar 5;9(1):942. doi: 10.1038/s41467-018-03340-7. 10.1038/s41467-018-03340-7 PubMed 29507340
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