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Addgene

pHelA12
(Plasmid #176493)

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This material is available to academics and nonprofits only. Orders shipped outside the U.S. may require additional regulatory approval, as well as a non-refundable export license fee of $85. Please log in to view availability.
Item Catalog # Description Quantity Price (USD)
Plasmid 176493 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5369
  • Total vector size (bp) 7147
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SARS-CoV NSP13
  • Species
    SARS-CoV
  • Mutation
    V570I
  • Entrez Gene
    ORF1ab (a.k.a. sarsp1)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 and T7 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer 5′-GGCGGATCCGCCATGGCTGTAGGTGCTTGTGTATTG-3′
  • 3′ sequencing primer 5′-GGCCTCGAGTCATTGTAATGTAGCCACATTGCG-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHelA12 was a gift from Xiaodong Cheng & Jian-Dong Huang (Addgene plasmid # 176493 ; http://n2t.net/addgene:176493 ; RRID:Addgene_176493)

  • For your References section:

    The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases. Tanner JA, Watt RM, Chai YB, Lu LY, Lin MC, Peiris JS, Poon LL, Kung HF, Huang JD. J Biol Chem. 2003 Oct 10;278(41):39578-82. doi: 10.1074/jbc.C300328200. Epub 2003 Aug 13. 10.1074/jbc.C300328200 PubMed 12917423
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