pET28a-SARS-CoV-2 NSP13
(Plasmid #176492)

• Purpose: Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.
• Depositing Lab: Xiaodong Cheng
• Publication: White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144-9151. doi: 10.1021/acs.jpclett.0c02421. Epub 2020 Oct 14.

Backbone

• Vector backbone: pET28a(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 7147
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SARS-CoV-2 NSP13
• Species: SARS-CoV
• Mutation: I570 from SARS-CoV NSP13 was mutated to V to convert it to SARS-CoV-2 NSP13
• Entrez Gene: ORF1ab (a.k.a. GU280_gp01)
• Promoter: T7
• Tag / Fusion Protein:
  • His6 and T7 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: 5′-GGCGGATCCGCCATGGCTGTAGGTGCTTGTGTATTG-3′
• 3′ sequencing primer: 5′-GGCCTCGAGTCATTGTAATGTAGCCACATTGCG-3′

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We received the clone for SARS-CoV NSP13 from Dr. Jiandong Huang from University of Hong Kong

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

The original vector pHelA12 was described in a paper published by Dr. Huang (PMID: 12917423). We mutated the I570 of the original SARS-CoV NSP13 to V to convert it to SARS-CoV-2 NSP13.