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Addgene

Flag-hCRM1
(Plasmid #17647)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17647 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3xFLAG CMV-10
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRM1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3300
  • Entrez Gene
    XPO1 (a.k.a. CRM-1, CRM1, emb, exp1)
  • Tag / Fusion Protein
    • 3x Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag-hCRM1 was a gift from Xin Wang (Addgene plasmid # 17647 ; http://n2t.net/addgene:17647 ; RRID:Addgene_17647)

  • For your References section:

    Temporal and spatial control of nucleophosmin by the Ran-Crm1 complex in centrosome duplication. Wang W, Budhu A, Forgues M, Wang XW. Nat Cell Biol. 2005 Aug . 7(8):823-30. 10.1038/ncb1282 PubMed 16041368
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