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Addgene

pCMV-Tag1-NS5A
(Plasmid #17646)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17646 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMVTag1
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HCV NS5A
  • Alt name
    NS5A
  • Alt name
    HCV non-structural (NS) protein 5A
  • Species
    Hepatitis C virus
  • Insert Size (bp)
    1400
  • Entrez Gene
    POLY (a.k.a. HCVgp1)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Tag1-NS5A was a gift from Xin Wang (Addgene plasmid # 17646 ; http://n2t.net/addgene:17646 ; RRID:Addgene_17646)

  • For your References section:

    Induction of a unique gene expression profile in primary human hepatocytes by hepatitis C virus core, NS3 and NS5A proteins. Budhu A, Chen Y, Kim JW, Forgues M, Valerie K, Harris CC, Wang XW. Carcinogenesis. 2007 Jul . 28(7):1552-60. 10.1093/carcin/bgm075 PubMed 17404395
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