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pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVI
(Plasmid #176450)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176450 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pVRC8400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y
  • Mutation
    K417N, E484K, N501Y
  • Entrez Gene
    S (a.k.a. GU280_gp02, spike glycoprotein)
  • Tags / Fusion Proteins
    • scFc (N terminal on insert)
    • HRV3C (N terminal on insert)
    • AVI (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVRC8400-SARS-CoV-2-RBD-SD1-K417N-E484K-N501Y-AVI was a gift from Peter Kwong (Addgene plasmid # 176450 ; http://n2t.net/addgene:176450 ; RRID:Addgene_176450)

  • For your References section:

    Molecular probes of spike ectodomain and its subdomains for SARS-CoV-2 variants, Alpha through Omicron. Teng IT, Nazzari AF, Choe M, Liu T, Oliveira de Souza M, Petrova Y, Tsybovsky Y, Wang S, Zhang B, Artamonov M, Madan B, Huang A, Lopez Acevedo SN, Pan X, Ruckwardt TJ, DeKosky BJ, Mascola JR, Misasi J, Sullivan NJ, Zhou T, Kwong PD. PLoS One. 2022 May 24;17(5):e0268767. doi: 10.1371/journal.pone.0268767. eCollection 2022. 10.1371/journal.pone.0268767 PubMed 35609088
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