pcDNA3-myc-his-BACH1 K52R
(Plasmid #17643)

• Depositing Lab: Ronny Drapkin
• Publication: Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 101(8):2357-62.

Backbone

• Vector backbone: pcDNA3.1(+)/myc-His A
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5493
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: BACH1
• Alt name: BRIP1
• Alt name: FANCJ
• Species: H. sapiens (human)
• Mutation: The lysine residue at position 52 was changed to an arginine, using a Quik-change site-directed mutagenesis kit (Stratagene).
• GenBank ID: AF360549
• Entrez Gene: BRIP1 (a.k.a. BACH1, FANCJ, OF)
• Tags / Fusion Proteins:
  • Myc (C terminal on backbone)
  • His (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Apa1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry