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Addgene

7MEQ
(Plasmid #176412)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176412 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFHMSP-LIC-C
  • Backbone manufacturer
    SGC (Addgene Plasmid #26100)
  • Vector type
    Baculovirus Expresssion

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TMPRSS2 – transmembrane serine protease 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1149
  • Mutation
    etmprss2_S251D/R252D/Q253D/S254D/R255K
  • Entrez Gene
    TMPRSS2 (a.k.a. PRSS10)
  • Promoter Polyhedrin
  • Tags / Fusion Proteins
    • Honeybee melittin signal (N terminal on insert)
    • 8 x His Tag (C terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7MEQ was a gift from Cheryl Arrowsmith (Addgene plasmid # 176412 ; http://n2t.net/addgene:176412 ; RRID:Addgene_176412)
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