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Addgene

GFP-Trim46
(Plasmid #176402)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176402 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ?pGW1-GFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    trim46
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Trim46 (a.k.a. TRIFIC)
  • Tag / Fusion Protein
    • GFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Trim46 was a gift from Casper Hoogenraad & Thomas Schwarz (Addgene plasmid # 176402 ; http://n2t.net/addgene:176402 ; RRID:Addgene_176402)

  • For your References section:

    TRIM46 Controls Neuronal Polarity and Axon Specification by Driving the Formation of Parallel Microtubule Arrays. van Beuningen SFB, Will L, Harterink M, Chazeau A, van Battum EY, Frias CP, Franker MAM, Katrukha EA, Stucchi R, Vocking K, Antunes AT, Slenders L, Doulkeridou S, Sillevis Smitt P, Altelaar AFM, Post JA, Akhmanova A, Pasterkamp RJ, Kapitein LC, de Graaff E, Hoogenraad CC. Neuron. 2015 Dec 16;88(6):1208-1226. doi: 10.1016/j.neuron.2015.11.012. Epub 2015 Dec 6. 10.1016/j.neuron.2015.11.012 PubMed 26671463
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