pfa6a mScarlet-TsCC(B)::KANMX
(Plasmid #176397)

• Purpose: Plasmid for cloning mScarlet fused to TsCC(B) coiled coil into yeast.
• Depositing Lab: Matthew Good
• Publication: Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007. doi: 10.1038/s41589-021-00840-4. Epub 2021 Aug 2.

Backbone

• Vector backbone: pfa6a::KAN
• Total vector size (bp): 5817
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mScarlet-TsCC(B)
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1641
• Promoter: none
• Tag / Fusion Protein:
  • mScarlet fused to TsCC(B) (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: none

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid is used for cloning and integrating PCR products into yeast genome.