pST39-His/Vps27(345-440) - Hse(275-375)
(Plasmid
#17638)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 17638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepST39
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pST39-His/Vps27(345-440) - Hse(275-375) was a gift from James Hurley (Addgene plasmid # 17638 ; http://n2t.net/addgene:17638 ; RRID:Addgene_17638) -
For your References section:
The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting. Prag G, Watson H, Kim YC, Beach BM, Ghirlando R, Hummer G, Bonifacino JS, Hurley JH. Dev Cell. 2007 Jun . 12(6):973-86. 10.1016/j.devcel.2007.04.013 PubMed 17543868