pGST2-Bro1 (1-387)
(Plasmid
#17635)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17635 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneparallel GST2
- Backbone size w/o insert (bp) 5000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBro1 (1-387)
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Alt nameBro1
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1160
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Entrez GeneBRO1 (a.k.a. YPL084W, ASI6, LPF2, NPI3, VPS31)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pGEX5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGST2-Bro1 (1-387) was a gift from James Hurley (Addgene plasmid # 17635 ; http://n2t.net/addgene:17635 ; RRID:Addgene_17635) -
For your References section:
Structural basis for endosomal targeting by the Bro1 domain. Kim J, Sitaraman S, Hierro A, Beach BM, Odorizzi G, Hurley JH. Dev Cell. 2005 Jun . 8(6):937-47. 10.1016/j.devcel.2005.04.001 PubMed 15935782