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Addgene

ESCRT-I
(Plasmid #17634)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17634 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST39
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vps23(1-385) – His/Vps28(1-242) – Vps37(1-213) – Mvb12(1-101)
  • Alt name
    Vps23
  • Alt name
    Vps28
  • Alt name
    Vps37
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    VPS28 (a.k.a. YPL065W, VPL13, VPT28)
  • Entrez Gene
    STP22 (a.k.a. YCL008C, AGS1, VPL15, VPS23)
  • Entrez Gene
    SRN2 (a.k.a. YLR119W, SRN10, VPL16, VPS37)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See comments (not destroyed)
  • 3′ cloning site See comments (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DNAs for Mvb12, Vps37, Vps28, and Vps23 were cloned into pST39.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ESCRT-I was a gift from James Hurley (Addgene plasmid # 17634 ; http://n2t.net/addgene:17634 ; RRID:Addgene_17634)

  • For your References section:

    Structural and functional organization of the ESCRT-I trafficking complex. Kostelansky MS, Sun J, Lee S, Kim J, Ghirlando R, Hierro A, Emr SD, Hurley JH. Cell. 2006 Apr 7. 125(1):113-26. 10.1016/j.cell.2006.01.049 PubMed 16615894
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