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Addgene

pSLfa-PUb-EGFP-3'UTR#5
(Plasmid #176337)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176337 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLfa
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3'UTR#5
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer AGAAGCGCGATCACATGGTCCTGC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLfa-PUb-EGFP-3'UTR#5 was a gift from Zach Adelman (Addgene plasmid # 176337 ; http://n2t.net/addgene:176337 ; RRID:Addgene_176337)

  • For your References section:

    Novel synthetic 3'-untranslated regions for controlling transgene expression in transgenic Aedes aegypti mosquitoes. Chae K, Valentin C, Jakes E, Myles KM, Adelman ZN. RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/15476286.2021.1971440. 10.1080/15476286.2021.1971440 PubMed 34464234
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