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Addgene

ESCRT-II
(Plasmid #17633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17633 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST39
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pST39-Vps25(1-203) - His/Vps22(1-234) - Vps36 (1-567)
  • Alt name
    Vps25
  • Alt name
    Vps22
  • Alt name
    Vps36
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    VPS36 (a.k.a. GRD12, VAC3, VPL11)
  • Entrez Gene
    SNF8 (a.k.a. VPL14, VPS22)
  • Entrez Gene
    VPS25 (a.k.a. VPL12, VPT25)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site See comments (not destroyed)
  • 3′ cloning site See comments (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DNAs for Vps25, Vps22, and Vps36 were cloned in order into pST39. Vps22 was tagged with an N-terminal hexahistidine tag and a TEV protease cleavage site; the other subunits were untagged.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ESCRT-II was a gift from James Hurley (Addgene plasmid # 17633 ; http://n2t.net/addgene:17633 ; RRID:Addgene_17633)

  • For your References section:

    Structure of the ESCRT-II endosomal trafficking complex. Hierro A, Sun J, Rusnak AS, Kim J, Prag G, Emr SD, Hurley JH. Nature. 2004 Sep 9. 431(7005):221-5. 10.1038/nature02914 PubMed 15329733
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