pGST1-Osh4 (2-434)
(Plasmid #17632)

• Depositing Lab: James Hurley
• Publication: Im et al Nature. 2005 Sep 1. 437(7055):154-8.

Backbone

• Vector backbone: modified pGEX-4T
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Osh4
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1300
• Entrez Gene: KES1 (a.k.a. YPL145C, BSR3, LPI3, OSH4)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: pGEX5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

DNA encoding Osh4 residues 2–434 was amplified by PCR and subcloned into the BamHI and XhoI sites of a pGEX-4T vector modified to contain a TEV protease recognition site.