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pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
(Plasmid #176311)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176311 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSin-EF1a
  • Backbone size w/o insert (bp) 6288
  • Total vector size (bp) 9696
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
  • Species
    Synthetic
  • Insert Size (bp)
    3408
  • Promoter human EF1alpha
  • Tag / Fusion Protein
    • tagBFP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ctcaagcctcagacagtggttcaa
  • 3′ sequencing primer gggagaggggcggaattggc
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR was a gift from Yingxiao Wang (Addgene plasmid # 176311 ; http://n2t.net/addgene:176311 ; RRID:Addgene_176311)

  • For your References section:

    An AND-Gated Drug and Photoactivatable Cre-loxP System for Spatiotemporal Control in Cell-Based Therapeutics. Allen ME, Zhou W, Thangaraj J, Kyriakakis P, Wu Y, Huang Z, Ho P, Pan Y, Limsakul P, Xu X, Wang Y. ACS Synth Biol. 2019 Oct 18;8(10):2359-2371. doi: 10.1021/acssynbio.9b00175. Epub 2019 Oct 8. 10.1021/acssynbio.9b00175 PubMed 31592660
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