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pGST2-Ub CPS (8-16)
(Plasmid #17631)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17631 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    parallel GST2
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ub CPS (8-16)
  • Alt name
    Ubi1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    RPL40A (a.k.a. YIL148W, CEP52A, UB11, UBI1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The vector backbone, parallel GST2, was made by replacing the polylinker of pGEX4T1 with the polylinker isolated from the baculovirus expression plasmid pFastBac (see PMID: 10024467).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST2-Ub CPS (8-16) was a gift from James Hurley (Addgene plasmid # 17631 ; http://n2t.net/addgene:17631 ; RRID:Addgene_17631)

  • For your References section:

    Structural basis for ubiquitin recognition and autoubiquitination by Rabex-5. Lee S, Tsai YC, Mattera R, Smith WJ, Kostelansky MS, Weissman AM, Bonifacino JS, Hurley JH. Nat Struct Mol Biol. 2006 Mar . 13(3):264-71. 10.1038/nsmb1064 PubMed 16462746
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