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Addgene

pMVS223
(Plasmid #176293)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176293 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMVS223
  • Backbone size (bp) 5897
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The Zynthetic Zinc Finger DNA Binding Domain is from Park, M., Patel, N., Keung, A. J. & Khalil, A. S. Engineering Epigenetic Regulation Using Synthetic Read-Write Modules. Cell 176, 227-238.e20 (2019).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMVS223 was a gift from Max Staller (Addgene plasmid # 176293 ; http://n2t.net/addgene:176293 ; RRID:Addgene_176293)

  • For your References section:

    Directed mutational scanning reveals a balance between acidic and hydrophobic residues in strong human activation domains. Staller MV, Ramirez E, Kotha SR, Holehouse AS, Pappu RV, Cohen BA. Cell Syst. 2022 Apr 20;13(4):334-345.e5. doi: 10.1016/j.cels.2022.01.002. Epub 2022 Feb 3. 10.1016/j.cels.2022.01.002 PubMed 35120642
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