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AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG
(Plasmid #176285)

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Item Catalog # Description Quantity Price (USD)
Plasmid 176285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TVA66T-dTomato-rabies Glycoprotein
  • Species
    Rabies Virus CVS N2c strain
  • Insert Size (bp)
    4400
  • Tag / Fusion Protein
    • dTomato (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AiP5003 pAAV-Syn-DIO-TVA66T-dTom-CVS-N2cG was a gift from The Allen Institute for Brain Science & Shenqin Yao (Addgene plasmid # 176285 ; http://n2t.net/addgene:176285 ; RRID:Addgene_176285)

  • For your References section:

    A whole-brain monosynaptic input connectome to neuron classes in mouse visual cortex. Yao S, Wang Q, Hirokawa KE, Ouellette B, Ahmed R, Bomben J, Brouner K, Casal L, Caldejon S, Cho A, Dotson NI, Daigle TL, Egdorf T, Enstrom R, Gary A, Gelfand E, Gorham M, Griffin F, Gu H, Hancock N, Howard R, Kuan L, Lambert S, Lee EK, Luviano J, Mace K, Maxwell M, Mortrud MT, Naeemi M, Nayan C, Ngo NK, Nguyen T, North K, Ransford S, Ruiz A, Seid S, Swapp J, Taormina MJ, Wakeman W, Zhou T, Nicovich PR, Williford A, Potekhina L, McGraw M, Ng L, Groblewski PA, Tasic B, Mihalas S, Harris JA, Cetin A, Zeng H. Nat Neurosci. 2023 Feb;26(2):350-364. doi: 10.1038/s41593-022-01219-x. Epub 2022 Dec 22. 10.1038/s41593-022-01219-x PubMed 36550293
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