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Addgene

iDuet011a
(Plasmid #17628)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 17628 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Duet011
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin ; GFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TetO7 - UBC promoter - GFP - PGK promoter - Hygro

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene).

Tet-Inducible Vector. The 309bp tetO7 sequence was PCR-amplified from the template pLVTH-siGFP. The inducible iDuet011A vector has the tetO7 sequence inserted at the PacI site (with a BstXI linker), immediately upstream of the UBC promoter

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    iDuet011a was a gift from Linzhao Cheng (Addgene plasmid # 17628 ; http://n2t.net/addgene:17628 ; RRID:Addgene_17628)

  • For your References section:

    Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer. Zhou BY, Ye Z, Chen G, Gao ZP, Zhang YA, Cheng L. Stem Cells. 2007 Mar . 25(3):779-89. 10.1634/stemcells.2006-0128 PubMed 17158240
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