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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 17627 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneDuet011
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin ; GFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUBC promoter - GFP - PGK promoter - Hygro
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Duet011 was a gift from Linzhao Cheng (Addgene plasmid # 17627 ; http://n2t.net/addgene:17627 ; RRID:Addgene_17627) -
For your References section:
Inducible and reversible transgene expression in human stem cells after efficient and stable gene transfer. Zhou BY, Ye Z, Chen G, Gao ZP, Zhang YA, Cheng L. Stem Cells. 2007 Mar . 25(3):779-89. 10.1634/stemcells.2006-0128 PubMed 17158240
Map uploaded by the depositor.
