pAAV-FLEX-P301L Tau
(Plasmid #176267)

• Purpose: This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.
• Depositing Lab: Tsuneya Ikezu
• Publication: You et al Neuroscience. 2019 Dec 1;422:65-74. doi: 10.1016/j.neuroscience.2019.10.001. Epub 2019 Nov 2.

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: pAAV-EF1a-FLEX-mCherry
• Backbone manufacturer: addgene
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Flex-P301L tau
• Entrez Gene: MAPT (a.k.a. DDPAC, FTD1, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, Tau-PHF6, tau-40)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Mlu1 (unknown if destroyed)
• 3′ cloning site: Nhel (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry