Skip to main content
Addgene

pNOC_dCas9-KRAB_sgRNA Td2
(Plasmid #176264)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176264 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pUC19
  • Vector type
    CRISPR, Luciferase, Synthetic Biology ; Expression in microalgae Nannochloropsis oceanica
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dead spCas9
  • Species
    Synthetic; Streptococcus pyogenes
  • Mutation
    H840A and D10A mutations on spCas9 to inactivate the endonuclease activity.
  • Tags / Fusion Proteins
    • luciferase (C terminal on insert)
    • Kruppel associated box domain (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The backbone of plasmid and Cas9 sequence was a gift from Eva Farre from Michigan State University.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNOC_dCas9-KRAB_sgRNA Td2 was a gift from John van der Oost (Addgene plasmid # 176264 ; http://n2t.net/addgene:176264 ; RRID:Addgene_176264)

  • For your References section:

    Comprehensive Genome Engineering Toolbox for Microalgae Nannochloropsis oceanica Based on CRISPR-Cas Systems. Naduthodi MIS, Sudfeld C, Avitzigiannis EK, Trevisan N, van Lith E, Alcaide Sancho J, D'Adamo S, Barbosa M, van der Oost J. ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 10.1021/acssynbio.1c00329. Epub 2021 Nov 18. 10.1021/acssynbio.1c00329 PubMed 34793143
Related items: