pNOC_dCas9_sgRNA NC
(Plasmid #176259)

• Purpose: pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and
• Depositing Lab: John van der Oost
• Publication: Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-3378. doi: 10.1021/acssynbio.1c00329. Epub 2021 Nov 18.

Backbone

• Vector backbone: pUC19
• Vector type: CRISPR, Luciferase, Synthetic Biology ; Expression in microalgae Nannochloropsis oceanica
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: dead spCas9
• Species: Synthetic; Streptococcus pyogenes
• Mutation: H840A and D10A mutations on spCas9 to inactivate the endonuclease activity.
• Tag / Fusion Protein:
  • luciferase (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pnoc_dcas9_sgrna-nc.gb
• A portion of this plasmid was derived from a plasmid made by: The backbone of plasmid and Cas9 sequence was a gift from Eva Farre from Michigan State University.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry