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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1
(Plasmid #176237)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176237 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pspCas9-3'UTR-Tetra-COM-Vector
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spCas9 and polymerase exo domain fusion
  • Species
    H. sapiens (human)
  • Entrez Gene
    CLCN5 (a.k.a. CLC5, CLCK2, ClC-5, DENT1, DENTS, NPHL1, NPHL2, XLRH, XRN, hCIC-K2)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pspCas9-5Exo-tetra-com-hCLCN5-sp-g1 was a gift from Baisong Lu (Addgene plasmid # 176237 ; http://n2t.net/addgene:176237 ; RRID:Addgene_176237)

  • For your References section:

    Targeting DNA polymerase to DNA double-strand breaks reduces DNA deletion size and increases templated insertions generated by CRISPR/Cas9. Yoo KW, Yadav MK, Song Q, Atala A, Lu B. Nucleic Acids Res. 2022 Mar 22. pii: 6552087. doi: 10.1093/nar/gkac186. 10.1093/nar/gkac186 PubMed 35323942
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