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Addgene

pET-GFP-9S [rpoA]
(Plasmid #176221)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176221 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GFP-9S [rpoA]
  • Species
    Synthetic
  • Insert Size (bp)
    1641
  • Promoter T7
  • Tag / Fusion Protein
    • His-GFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-GFP-9S [rpoA] was a gift from Ian Small (Addgene plasmid # 176221 ; http://n2t.net/addgene:176221 ; RRID:Addgene_176221)

  • For your References section:

    Cofactor-independent RNA editing by a synthetic S-type PPR protein. Bernath-Levin K, Schmidberger J, Honkanen S, Gutmann B, Sun YK, Pullakhandam A, Colas des Francs-Small C, Bond CS, Small I. Synth Biol (Oxf). 2021 Dec 23;7(1):ysab034. doi: 10.1093/synbio/ysab034. eCollection 2021. 10.1093/synbio/ysab034 PubMed 35128071
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