pETx-SR6v15
(Plasmid
#176212)
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PurposeBacterial expression of the SR6v15 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 176212 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepETx
- Backbone size w/o insert (bp) 5639
- Total vector size (bp) 6014
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSR6v15
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SpeciesSynthetic
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Insert Size (bp)378
- Promoter T7
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Tag
/ Fusion Protein
- 6XHis (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GTTTAACTTTAAGAAGGAGATATACC
- 3′ sequencing primer TCCTTTCGGGCTTTGTTAGCAGCCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pETx-SR6v15 was a gift from Aviv Regev (Addgene plasmid # 176212 ; http://n2t.net/addgene:176212 ; RRID:Addgene_176212) -
For your References section:
A cell-free nanobody engineering platform rapidly generates SARS-CoV-2 neutralizing nanobodies. Chen X, Gentili M, Hacohen N, Regev A. Nat Commun. 2021 Sep 17;12(1):5506. doi: 10.1038/s41467-021-25777-z. 10.1038/s41467-021-25777-z PubMed 34535642
