CAG.mCherry-INBox(AAA)-eDHFR
(Plasmid
#176207)
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PurposeINBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176207 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEM791
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-INBoxAAA-eDHFR
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Alt nameINBox TSS mutant
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SpeciesH. sapiens (human)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG.mCherry-INBox(AAA)-eDHFR was a gift from Michael Lampson (Addgene plasmid # 176207 ; http://n2t.net/addgene:176207 ; RRID:Addgene_176207) -
For your References section:
Tension promotes kinetochore-microtubule release by Aurora B kinase. Chen GY, Renda F, Zhang H, Gokden A, Wu DZ, Chenoweth DM, Khodjakov A, Lampson MA. J Cell Biol. 2021 Jun 7;220(6). pii: 212027. doi: 10.1083/jcb.202007030. 10.1083/jcb.202007030 PubMed 33904910