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Addgene

CAG.3xHalo-GFP-SPC25
(Plasmid #176206)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 176206 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEM791
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xHalo-GFP-SPC25
  • Alt name
    SPC25 anchor
  • Species
    H. sapiens (human)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG.3xHalo-GFP-SPC25 was a gift from Michael Lampson (Addgene plasmid # 176206 ; http://n2t.net/addgene:176206 ; RRID:Addgene_176206)

  • For your References section:

    Tension promotes kinetochore-microtubule release by Aurora B kinase. Chen GY, Renda F, Zhang H, Gokden A, Wu DZ, Chenoweth DM, Khodjakov A, Lampson MA. J Cell Biol. 2021 Jun 7;220(6). pii: 212027. doi: 10.1083/jcb.202007030. 10.1083/jcb.202007030 PubMed 33904910
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