pMCL9
(Plasmid
#176186)
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Purpose(Empty Backbone) 2 mu cloning vector for single or multiplexed gRNAs, SNR52p-sfGFP-scRNA-SUP4t-CYC1t
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAssembly of 6 BsaI-digested fragments
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). 5' cloning site: BsaI (destroyed during cloning), 3' cloning site: BsaI (destroyed during cloning).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCL9 was a gift from Florian David (Addgene plasmid # 176186 ; http://n2t.net/addgene:176186 ; RRID:Addgene_176186) -
For your References section:
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries. Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F. ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 10.1021/acssynbio.1c00408. Epub 2021 Dec 3. 10.1021/acssynbio.1c00408 PubMed 34860007