pMCL8_28bp
(Plasmid
#176185)
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Purpose(Empty Backbone) plasmid containing scaffold gRNA for Cas9 followed by a 28bp Csy4 recognition sequence, RPR1p-tetO-GFP-scRNA-28bpCsy4-RPR1t
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176185 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMCL8
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMCL8_28bp was a gift from Florian David (Addgene plasmid # 176185 ; http://n2t.net/addgene:176185 ; RRID:Addgene_176185) -
For your References section:
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries. Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F. ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 10.1021/acssynbio.1c00408. Epub 2021 Dec 3. 10.1021/acssynbio.1c00408 PubMed 34860007