pMC8b-X4up
(Plasmid
#176164)
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Purposeyeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepYTK001
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5' homology for integration site X-4
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMC8b-X4up was a gift from Florian David (Addgene plasmid # 176164 ; http://n2t.net/addgene:176164 ; RRID:Addgene_176164) -
For your References section:
Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries. Otto M, Skrekas C, Gossing M, Gustafsson J, Siewers V, David F. ACS Synth Biol. 2021 Dec 17;10(12):3461-3474. doi: 10.1021/acssynbio.1c00408. Epub 2021 Dec 3. 10.1021/acssynbio.1c00408 PubMed 34860007