pLV-SspB-HaloTag-p63DH
(Plasmid
#176129)
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PurposeHeterodimerization with iLID, RhoA activation
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 176129 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLV
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRHOGEF p63
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Alt nameARHGEF25
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SpeciesH. sapiens (human)
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Entrez GeneARHGEF25 (a.k.a. GEFT, p63RhoGEF)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.10.17.512253 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLV-SspB-HaloTag-p63DH was a gift from Dorus Gadella (Addgene plasmid # 176129 ; http://n2t.net/addgene:176129 ; RRID:Addgene_176129) -
For your References section:
Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength. Mahlandt EK, Palacios Martinez S, Arts JJG, Tol S, van Buul JD, Goedhart J. Elife. 2023 Jul 14;12:RP84364. doi: 10.7554/eLife.84364. 10.7554/eLife.84364 PubMed 37449837